Goat anti-PLRG1 Antibody |
| Principle Name | PLRG1 |
| Official Symbol | PLRG1 |
| All Name and Symbols | PLRG1; pleiotropic regulator 1 (PRL1 homolog, Arabidopsis); PRL1; MGC110980; pleiotropic regulator 1 (PRL1, Arabidopsis homolog); pleiotropic regulator 1 (PRL1homolog, Arabidopsis); pleiotropic regulator 1 (PRL1, Arabidopsis homolog) |
| Accession Number(s) | NP_002660.1; NP_001188493.1 |
| Peptide Sequence | PVSWKPEIIKRKRF, is from C Terminus |
| Human Gene ID(s) | 5356 |
| Non-Human GeneID(s) | 60376 (rat); |
| Important Comments | This antibody is expected to recognise isoform 1 (NP_002660.1) and isoform 2 (NP_001188493.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 60kDa band observed in nuclear lysates of cell lines HEK293 and NIH3T3 (calculated MW of 57.2kDa according to Human NP_002660.1 and 56.9kDa according to Mouse NP_058064.2). Recommended concentration |
| Reference(s) | Ajuh P, Lamond AI Identification of peptide inhibitors of pre-mRNA splicing derived from the
essential interaction domains of CDC5L and PLRG1 Nucleic Acids Res. 2003 Nov 1;31(21) |
