Goat anti-NFIL3 Antibody |
| Principle Name | NFIL3 |
| Official Symbol | NFIL3 |
| All Name and Symbols | NFIL3; nuclear factor, interleukin 3 regulated; E4BP4; IL3BP1; NFIL3A; NF-IL3A; interleukin-3 promoter transcriptional activator; OTTHUMP00000021633 |
| Accession Number(s) | NP_005375.2 |
| Peptide Sequence | RLIATQPISASDSG, is from C Terminus |
| Human Gene ID(s) | 4783 |
| Non-Human GeneID(s) | 18030 (mouse); 114519 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Approx 50-55kDa band seen in lysates of cell line Daudii (calculated MW of 51.5kDa according to NP_005375.2). Primary incubation was 1 hour. Recommended for use at 1-3µg/ml. |
| Reference(s) | Zhang W, Zhang J, Kornuc M, Kwan K, Frank R, Nimer SD. Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter. Mol Cell Biol 1995 Nov;15(11) |
