Goat anti-MELK Antibody |
| Principle Name | MELK |
| Official Symbol | MELK |
| All Name and Symbols | MELK; maternal embryonic leucine zipper kinase; KIAA0175; RP11-8N6.1; HPK38; OTTHUMP00000046113; pEg3 kinase |
| Accession Number(s) | NP_055606.1 |
| Peptide Sequence | KRLVEDILSSCKV, is from C Terminus |
| Human Gene ID(s) | 9833 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Experiments gave bands at approx 75kDa and 150kDa in Human Testis lysates and in cell lines HeLa and Jurkat after 0.1µg/ml antibody staining. The150kDa band is explained by glycosylation of this member of the AMPK family. This protein has a |
| Reference(s) | Nagase T, Seki N, Ishikawa K, Tanaka A, Nomura N. Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res 1996 Feb 29;3(1) |
