Product Name | 1F8 Chagas Recombinant |
Synonyms | |
Description | Recombinant Chagas 1F8 protein (Epitope of the flagellar calcium-binding protein FCABP of Trypanosoma cruzi) produced in E.Coli, is a polypeptide chain having a total of 234 a.a (includes his tag). The protein is fused to a 6-His tag having a total Mw of 25.8kDa and a theoretical pI of 5.39. |
Uniprot Accesion Number | P07749 |
Amino Acid Sequence |
Source | Escherichia Coli. |
Physical Appearance and Stability | Store lyophilized 1F8 Chagas antigen at 2-8°C. After reconstitution, store at -20°C. Prevent freeze/thaw cycles. |
Formulation and Purity | 1F8 Chagas antigen was lyophilized from 20mM Hepes, 300mM NaCl, pH-8, and varying 284mM imidazole. Protein is >90% pure as determined by SDS PAGE. |
Application | Reacts with sera from infected patients, Useful for immunoassays. |
Solubility | It is recommended to reconstitute the lyophilized 1F8 Chagas protein in sterile distilled pyrogen free water at 100µg/ml, which can then be further diluted to other aqueous solutions. |
Biological Activity | |
Shipping Format and Condition | Lyophilized powder at room temperature. |
Usage Statement | Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |