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Trypsin-1 Human Recombinant


Product Name Trypsin-1 Human Recombinant
Synonyms Protease Serine 1 (Trypsin 1), TRY1, Cationic Trypsinogen,  TRP1, TRYP1, TRY4, beta-trypsin, Digestive Zymogen, Nonfunctional Trypsin 1, Trypsinogen 1, Trypsinogen A, EC 3.4.21.4.
Description Trypsin-1 Human Recombinant produced in CHO cells is a 24.1kDa single polypeptide chain consisting of 224 amino acids.
Uniprot Accesion Number P07477
Amino Acid SequenceIVGGYNCEEN SVPYQVSLNS GYHFCGGSLI NEQWVVSAGH CYKSRIQVRL GEHNIEVLEG NEQFINAAKI IRHPQYDRKT LNNDIMLIKL SSRAVINARV STISLPTAPP ATGTKCLISG WGNTASSGAD YPDELQCLDA PVLSQAKCEA SYPGKITSNM FCVGFLEGGK DSCQGDSGGP VVCNGQLQGV VSWGDGCAQK NKPGVYTKVY NYVKWIKNTI AANS.
Source Chinese Hamster Ovary cells.
Physical Appearance and Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity The Trypsin-1 filtered 0.22um solution contains 20mM TRIS-HCl buffer pH 7.2. Greater than 95% as determined by SDS-PAGE.
Application
Solubility
Biological Activity 1 unit hydrolyzes 1 µmole of Na-Benzoyl-D,L-arginine 4-nitroanilide (BANI) per minute at 37°C, pH 8.0, in the presence of 10mM calcium. The specific activity is >1,000 Units per mg protein as measured under the described conditions. 
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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