Listeriolysin-O PEST free Recombinant

Item#:

rAP-3543

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Product Name	Listeriolysin-O PEST free Recombinant
Synonyms	Listeriolysin-O, LLO, hlyA.
Description	Recombinant Listeriolysin O lacking both the signal secretion sequence and the PEST- like sequence. LLO-PEST minus is composed of 471 amino acids, starting from amino acid 60 to amino acid 529, with the addition of a methionine in its NH2-terminus.
Uniprot Accesion Number	Q9L5B9
Amino Acid Sequence
Source	Escherichia Coli.
Physical Appearance and Stability	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Formulation and Purity	The protein contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% glycerol and 0.5M NaCl. Greater than 90.0% as determined by SDS-PAGE.
Application	a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA (4). b. Production of specific monoclonal antibodies (5). c. Detection of anti-listeriolysin O antibodies (6).
Solubility
Biological Activity	7x104 HU/mg. 2mM DTT could be use to reactivate the toxin.
Shipping Format and Condition	Lyophilized powder at room temperature.
Usage Statement	Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.