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Listeriolysin-O PEST free Recombinant

Product Name Listeriolysin-O PEST free Recombinant
Synonyms Listeriolysin-O, LLO, hlyA.
Description Recombinant Listeriolysin O lacking both the signal secretion sequence and the PEST- like sequence. LLO-PEST minus is composed of 471 amino acids, starting from amino acid 60 to amino acid 529, with the addition of a methionine in its NH2-terminus.
Uniprot Accesion Number Q9L5B9

Amino Acid Sequence

Source Escherichia Coli.
Physical Appearance and Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Formulation and Purity The protein contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% glycerol and 0.5M NaCl. Greater than 90.0% as determined by SDS-PAGE.
Application a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA (4). b. Production of specific monoclonal antibodies (5). c. Detection of anti-listeriolysin O antibodies (6).
Biological Activity 7x104 HU/mg. 2mM DTT could be use to reactivate the toxin.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.