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Platelet Factor-4 Human Recombinant (CXCL4)
Product Name Platelet Factor-4 Human Recombinant (CXCL4)
Synonyms CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.
Description CXCL4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 7.8 kDa.
Uniprot Accesion Number P02776
Amino Acid SequenceThe sequence of the first four N-terminal amino acids was determined and was found to be Glu-Ala-Glu-Glu-Asp.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized CXCL4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL4 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The CXCL4 protein was lyophilized after extensive dialysis against 50mM Tris-HCl pH 8.0 and 150mM NaCl buffer. Greater than 95.0% as determined by
Application
Solubility It is recommended to reconstitute the lyophilized CXCL4 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50 of CXCL4 as determined by its ability to inhibit human FGF basic dependent proliferation of NR6R3T3 mouse fibroblasts was found to be 5-15 µg/ml.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.

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