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High-Mobility Group Box 1 Human Recombinant
Product Name High-Mobility Group Box 1 Human Recombinant
Synonyms HMG1, HMG3, SBP-1, Amphoterin, HMGB1, High-Mobility Group Box 1.
Description HMG1 Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 223 amino acids and having a molecular mass of 26 kDa.The HMGB-1 is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P09429
Amino Acid SequenceMGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDELEHHHHHH.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized HMGB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HMGB1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The HMG1 (1mg/ml) was lyophilized after extensive dialyses against 1x PBS pH-7.4. Greater than 95.0% as determined by
Application
Solubility It is recommended to reconstitute the lyophilized HMGB1 in sterile 18MO-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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