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Heat Shock Protein 90kDa Beta (GRP94) Member 1 Human Recombinant, HEK
Product Name Heat Shock Protein 90kDa Beta (GRP94) Member 1 Human Recombinant, HEK
Synonyms ECGP, GP96, TRA1, GRP94, HSP90B1, Endoplasmin, Heat shock protein 90 kDa beta member 1, 94 kDa glucose-regulated protein, gp96 homolog, Tumor rejection antigen 1.
Description HSP90B1 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Asp22-Glu798) containing a total of 789 amino acids, having a calculated molecular mass of 90.9kDa. HSP90B1 is fused to a 2 aa N-terminal linker, a 4 aa C-terminal linker and a 6 aa His tag at C-Terminus.
Uniprot Accesion Number P14625
Amino Acid SequenceASDDEVDVDG TVEEDLGKSR EGSRTDDEVV QREEEAIQLD GLNASQIREL REKSEKFAFQ AEVNRMMKLI INSLYKNKEI FLRELISNAS DALDKIRLIS LTDENALSGN EELTVKIKCD KEKNLLHVTD TGVGMTREEL VKNLGTIAKS GTSEFLNKMT EAQEDGQSTS ELIGQFGVGF YSAFLVADKV IVTSKHNNDT QHIWESDSNE FSVIADPRGN TLGRGTTITL VLKEEASDYL ELDTIKNLVK KYSQFINFPI YVWSSKTETV EEPMEEEEAA KEEKEESDDE AAVEEEEEEK KPKTKKVEKT VWDWELMNDI KPIWQRPSKE VEEDEYKAFY KSFSKESDDP MAYIHFTAEG EVTFKSILFV PTSAPRGLFD EYGSKKSDYI KLYVRRVFIT DDFHDMMPKY LNFVKGVVDS DDLPLNVSRE TLQQHKLLKV IRKKLVRKTL DMIKKIADDK YNDTFWKEFG TNIKLGVIED HSNRTRLAKL LRFQSSHHPT DITSLDQYVE RMKEKQDKIY FMAGSSRKEA ESSPFVERLL KKGYEVIYLT EPVDEYCIQA LPEFDGKRFQ NVAKEGVKFD ESEKTKESRE AVEKEFEPLL NWMKDKALKD KIEKAVVSQR LTESPCALVA SQYGWSGNME RIMKAQAYQT GKDISTNYYA SQKKTFEINP RHPLIRDMLR RIKEDEDDKT VLDLAVVLFE TATLRSGYLL PDTKAYGDRI ERMLRLSLNI DPDAKVEEEP EEEPEETAED TTEDTEQDED EEMDVGTDEE ETAKESTAE G PKLHHHHHH.
Source HEK 293.
Physical Appearance and Stability Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
Formulation and Purity HSP90B1 was filtered (0.4 µm) and lyophilized in phosphate buffered saline (PBS) pH 8.0, 1% (w/v) Sucrose and 4% (w/v) Mannitol. Greater than 90.0% as determined by SDS-PAGE.
Application
Solubility It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. HSP90B1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Biological Activity
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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