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Glycoprotein Nmb Human Recombinant, HEK
Product Name Glycoprotein Nmb Human Recombinant, HEK
Synonyms Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, HGFIN, NMB.
Description GPNMB Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (a.a 22-486) containing a total of 477 amino acids, having a molecular mass of 53.7kDa (calculated), though it migrates at approximately 97kDa on SDS PAGE, the GPNMB is fused to a 2 a.a C-terminal linker and a 10 a.a His tag at C-Terminus.The Human GPNMB is purified by proprietary chromatographic techniques.
Uniprot Accesion Number Q14956
Amino Acid SequenceAKRFHDVLGN ERPSAYMREH NQLNGWSSDE NDWNEKLYPV WKRGDMRWKN SWKGGRVQAV LTSDSPALVG SNITFAVNLI FPRCQKEDAN GNIVYEKNCR NEAGLSADPY VYNWTAWSED SDGENGTGQS HHNVFPDGKP FPHHPGWRRW NFIYVFHTLG QYFQKLGRCS VRVSVNTANV TLGPQLMEVT VYRRHGRAYV PIAQVKDVYV VTDQIPVFVT MFQKNDRNSS DETFLKDLPI MFDVLIHDPS HFLNYSTINY KWSFGDNTGL FVSTNHTVNH TYVLNGTFSL NLTVKAAAPG PCPPPPPPPR PSKPTPSLAT TLKSYDSNTP GPAGDNPLEL SRIPDENCQI NRYGHFQATI TIVEGILEVN IIQMTDVLMP VPWPESSLID FVVTCQGSIP TEVCTIISDP TCEITQNTVC SPVDVDEMCL LTVRRTFNGS GTYCVNLTLG DDTSLALTST LISVP KLHHH HHHHHHH.
Source HEK 293.
Physical Appearance and Stability Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
Formulation and Purity Filtered (0.4µm) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4. Greater than 95.0% as determined by SDS-PAGE.
Application
Solubility It is recommended to add deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. GPNMB is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Biological Activity
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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