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Cystatin-C Human Recombinant, Active
Product Name Cystatin-C Human Recombinant, Active
Synonyms Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328.
Description Cystatin-C Human Recombinant produced in HEK cells is a non-glycosylated monomer, having a molecular weight of approximately 13kDa.The Cystatin-C is purified by proprietary chromatographic techniques.
Uniprot Accesion Number Q6FGW9

Amino Acid Sequence

Source HEK.
Physical Appearance and Stability Lyophilized Cystatin-C although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Cystatin-C should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The Cystatin-C was lyophilized from 1mg/ml in 1xPBS. Greater than 95% as obsereved by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized Cystatin-C in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The inhibitory function of Cystatin-C on papin¿s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5-20 µg/ml (0.3-1.5 µM) with a range of 1.56-50µg/ml Cystatin-C in presence of 0.55µM papain and 0.44µM L-BAPA. The activity is typically 0.3-1.5µM IC50.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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