B-Cell Lymphoma Extra Large Human Recombinant, GST

Item#:

rAP-2842

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Product Name	B-Cell Lymphoma Extra Large Human Recombinant, GST
Synonyms	BclXL, Bcl-X(L), Bcl-XL.
Description	Bcl-XL Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 1-210.The Bcl-XL is expressed as GST-Tag fusion protein and purified by proprietary chromatographic techniques.
Uniprot Accesion Number
Amino Acid Sequence
Source	Escherichia Coli.
Physical Appearance and Stability	Lyophilized Bcl-XL although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-XL should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity	The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl. Greater than 95.0% as determined by SDS-PAGE.
Application
Solubility	Suspend BclXL in 100?l of 0.5M Acetic acid, over night at 4°C. Dilute 10 fold into selected buffer system.Bcl-XL has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
Biological Activity
Shipping Format and Condition	Lyophilized powder at room temperature.
Usage Statement	Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.