Product Name | Actin |
Synonyms | |
Description | Ultra pure Actin consists in the alpha-skeletal muscle isoform and is purified from rabbit striated muscle.The purification method used (according to Spudich & Watts) results in a highly purified protein having a Molecular mass of 43,000 dalton. |
Uniprot Accesion Number | |
Amino Acid Sequence |
Source | Rabbit Muscle. |
Physical Appearance and Stability | Lyophilized Actin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Actin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
Formulation and Purity | The protein was lyophilized from a 1mg/ml solution containing 10mM Tris/HCl buffer pH 8.0, 0.2mM CaCl2, 0.2mM ATP, 1mM DTT and 0.5% (w/v) SDS. Greater than 98.0% as determined by SDS-PAGE. |
Application | |
Solubility | It is recommended to reconstitute the lyophilized Actin in sterile 18MΩ-cm H2O not less than 1mg/ml, which can then be further diluted to other aqueous solutions. |
Biological Activity | |
Shipping Format and Condition | Lyophilized powder at room temperature. |
Usage Statement | Our products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |