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Transforming Growth Factor-Alpha Human Recombinant
Product Name Transforming Growth Factor-Alpha Human Recombinant
Synonyms Transforming Growth Factor Alpha, Protransforming Growth Factor Alpha, TGF-Alpha, TGFA.
Description TGFA Human Recombinant (40-89) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 6kDa.The TGFA is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P01135
Amino Acid SequenceVVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized TGFA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFA should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity Lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA. Greater than 97.0% as determined by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized TGFA in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50, as measured in a proliferation assay using mouse Balb/3T3 cells, is less than 0.4ng/ml.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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