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Placenta Growth Factor-1 Human Recombinant


Product Name Placenta Growth Factor-1 Human Recombinant
Synonyms PIGF, PGF, PLGF-1, PlGF-2.
Description PLGF1 Human Recombinant (21-221 a.a.) produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 129 amino acids and having a molecular mass of 29kDa.The PLGF-1 Human Recombinant protein is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P49763
Amino Acid SequenceAVPPQQWALS AGNGSSEVEV VPFQEVWGRS YCRALERLVD VVSEYPSEVE HMFSPSCVSL LRCTGCCGDE NLHCVPVETA NVTMQLLKIR SGDRPSYVEL TFSQHVRCEC RPLREKMKPE RCGDAVPRR.
Source Escherichia coli.
Physical Appearance and Stability Lyophilized PLGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Formulation and Purity PLGF-1 protein was lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA. Greater than 97.0% as determined by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized PLGF1 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity Binds to immobilized rHuVEGFR1 with EC50 less than 6ng/ml.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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