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Platelet-derived Growth Factor AA Mouse Recombinant
Product Name Platelet-derived Growth Factor AA Mouse Recombinant
Synonyms Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.
Description PDGF-AA Mouse Recombinant is a disulfide linked homodimeric, non-glycosylated, polypeptide chain containing 2 x 126 amino acids and having a total molecular mass of 28.9 kDa. PDGF-AA is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P20033
Amino Acid SequenceMSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The protein was lyophilized with no additives. Greater than 97.0% as determined by
Application
Solubility It is recommended to reconstitute the lyophilized PDGF-AA in sterile 18MO-cm H2O at a concentration ranging between 0.1-0.5mg per 1ml, which can then be further diluted to other aqueous solutions.
Biological Activity Established by the dose-dependent stimulation of Balb/c 3T3 cells proliferation. The expected ED50  for this effect is 8-10 ng/ml corresponding to a specific activity of 100,000-125,000IU/mg.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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