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Platelet-derived Growth Factor AA Human Recombinant, Yeast


Item#:
rAP-2419
Size Price Quantity
2.0 ug $70.00
10.0 ug $182.00
1000.0 ug $6,552.00
Product Name Platelet-derived Growth Factor AA Human Recombinant, Yeast
Synonyms Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.
Description PDGF-AA Human Recombinant produced in Yeast is a homodimeric, glycosilated, polypeptide chain containing 2 x 110 amino acids and having a total molecular mass of 34 kDa. PDGF-AA is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P04085

Amino Acid Sequence

Source Pichia Pastoris.
Physical Appearance and Stability Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The protein was lyophilized with 20mM sodium phosphate buffer. Greater than 98.0% as determined by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized PDGF-AA in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50 was found to be 1ng/ml  corresponding to a Specific Activity of 1,000,000IU/mg calculated by the ability to stimulate the proliferation of mouse 3T3 fibroblasts (PNAS 94, 10205, 1997. Biochemistry, 1996, 35, 12077).
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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