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Platelet-Derived Growth Factor AA Human Recombinant
Product Name Platelet-Derived Growth Factor AA Human Recombinant
Synonyms Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.
Description Platelet-Derived Growth Factor AA Human Recombinant is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 125 amino acids and having a total molecular mass of 28511 Dalton. PDGF-AA is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P04085
Amino Acid SequenceSIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized Platelet-Derived Growth Factor AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity Lyophilized without any additives. Greater than 95.0% as determined by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AA in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50, calculated by the dose-dependent proliferation of murine 3T3 indicator cells is < 0.32ng/ml, corresponding to a Specific Activity of 3,125x103units/mg.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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