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Osteoprotegerin Human Recombinant, His Tag


Product Name Osteoprotegerin Human Recombinant, His Tag
Synonyms TNFRSF11B, OPG, OCIF, Osteoclastogenesis inhibitory factor, TR1, MGC29565.
Description Recombinant Human OCIF produced in E.coli cells is a single, non-glycosylated, polypeptide chain containing amino acids 201-401 and having a molecular mass of 31 kDa which includes a 4 kDa His tag.The OPG is purified by proprietary chromatographic techniques.
Uniprot Accesion Number O00300

Amino Acid Sequence

Source Escherichia Coli.
Physical Appearance and Stability Lyophilized Osteoprotegerin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution OCIF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1mM DTT. Greater than 80.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Application 1. Positive control for Western blot.2. Antibody production. 3. Protein assay.
Solubility It is recommended to reconstitute the lyophilized Osteoprotegerin in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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