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Insulin-Like Growth Factor 1 Rat Recombinant
Product Name Insulin-Like Growth Factor 1 Rat Recombinant
Synonyms Somatomedin C, IGF-I, IGFIA, IGF1.
Description Insulin-Like Growth Factor I Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.7kDa. IGF-I is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P08025
Amino Acid SequenceGPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized IGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFI should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The protein was lyophilized with a 0.2µm filtered concentrated solution in 20mM PBS, pH 7.0. Greater than 98.0% as determined by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized IGF1 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50 as determined by a cell proliferation assay using FDC-P1 cells is less than 2.0ng/ml, corresponding to a specific activity of >500,000units/mg.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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