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Fibroblast Growth Factor-basic Human Recombinant, Thermostable

Size Price Quantity
2.0 ug $50.00
10.0 ug $130.00
1000.0 ug $4,200.00
Product Name Fibroblast Growth Factor-basic Human Recombinant, Thermostable
Synonyms Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Description FGF-Basic Thermostable is a stabilized form of FGF-2 growth factor which enables a novel method to produce FGF2-dependent cell cultures more efficiently, having less media changes. FGF-Basic Thermostable can maintain its biological activity even after five days at 37C. The increase in the stability of FGF2 in cell-culture enables a more homogenous, undifferentiated stem cell culture, while saving scientists crucial time and money, as frequent supplementation of FGF-basic and the everyday medium change is not necessary. Thermostable FGF-2 is a hyperstable protein. The Thermal stability of the protein is increased by 15C compared to the wild-type FGF-2. Thermostable FGF-2 is more than 5-times prolonged half-life in human cell culture incubated at 37C. The FGF-2 Thermostable protein is engineered with fully retained biological function and has no harmful stabilizing additives.Thermostable FGF2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids including a 20 aa His tag and thrombin cleavage recognition site at N-terminus. The total calculated molecular mass is 19.4kDa.
Uniprot Accesion Number P09038

Amino Acid Sequence

Source Escherichia Coli.
Physical Appearance and Stability Store lyophilized protein at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4C for a limited period of time; it does not show any change after two weeks at 4C.
Formulation and Purity FGF2 was filtered and lyophilized from a solution containing 25mM potassium phosphate buffer and 300mM sodium chloride, pH 8.0 Purity is greater than 95% as determined by SDS-PAGE.
Solubility It is recommended to add deionized water to prepare a working stock solution of approximately 0.5-1mg/ml and let the lyophilized pellet dissolve completely.
Biological Activity
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.