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Fibroblast Growth Factor-8 Human Recombinant
Product Name Fibroblast Growth Factor-8 Human Recombinant
Synonyms FGF8B, FGF-8B, FGF8-B, KAL6, HBGF-8, HBGF8, AIGF, HBGF-8, MGC149376, fibroblast growth factor 8.
Description FGF 8 Human Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 194 amino acids and having a total molecular mass of 22.5kDa. 
Uniprot Accesion Number P55075
Amino Acid SequenceMQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGDPFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized FGF 8 although stable at room temperature for 3 weeks, should be stored desiccated below -18?C. Upon reconstitution FGF 8 should be stored at 4?C between 2-7 days and for future use below -18?C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity Lyophilized from a concentrated (1mg/ml) solution containing 5mM Na3PO4 and 50mM NaCl, pH 7.5. Greater than 97.0% as determined by analysis by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized FGF 8 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. 
Biological Activity The ED50, as determined by its ability to induce proliferation of mouse 3T3 fibroblasts, is 5-7ng/ml, corresponding to a specific activity of 2x105units/mg.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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