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Fibroblast Growth Factor-21 Human Recombinant, His Tag
Product Name Fibroblast Growth Factor-21 Human Recombinant, His Tag
Synonyms Fibroblast growth factor 21, FGF-21.
Description Fibroblast Growth Factor -21 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 202 amino acids (29-209) and having a molecular mass of 21.6 kDa (molecular weight on SDS-PAGE will appear higher). The FGF-21 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
Uniprot Accesion Number Q9NSA1
Amino Acid SequenceMGSSHHHHHH SSGLVPRGSH MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVKTSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPAPPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS.
Source Escherichia Coli.
Physical Appearance and Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Formulation and Purity The FGF-21 His tag protein (1mg/ml) solution in 20mM Tris-HCl buffer pH-8 and 10% glycerol. Greater than 90.0% as determined by SDS-PAGE.
Application
Solubility
Biological Activity Measured in a cell proliferation assay using NIH-3T3 cells. The ED50 for this effect is <12ng/ml, which corresponds to > 83,000 units/ml.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.

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