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Macrophage-Colony Stimulating Factor Rat Recombinant
Product Name Macrophage-Colony Stimulating Factor Rat Recombinant
Synonyms Macrophage colony-stimulating factor 1, CSF-1, MCSF, Csf1, Csfm.
Description Macrophage Colony Stimulating Factor Rat Recombinant produced in E.coli is a non-glycosylated homodimer, containing 2 x 155 amino acids and having a total molecular mass of 36.2 kDa.MCSF is purified by proprietary chromatographic techniques.
Uniprot Accesion Number Q8JZQ0
Amino Acid SequenceMEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF  FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity Lyophilized from a sterile filtered aqueous solution containing 10mM Na3PO4, pH 7.5. Greater than 95.0% as determined by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized MCSF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The activity is determined by dose-dependent induction of M-NFS-60 cell proliferation and is typically less than 2 ng/ml. This corresponds to an expected specific activity of 5x105 units/mg.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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