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Granulocyte Macrophage-Colony Stimulating Factor Canine Recombinant
Product Name Granulocyte Macrophage-Colony Stimulating Factor Canine Recombinant
Synonyms CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, MGC131935, MGC138897.
Description GMCSF k9 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14.2 kDa. GM-CSF is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P48749
Amino Acid SequenceAPTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPEGPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQNINFKSFKENL KDFLFNIPFD CWKPVKK.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized GMCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity GMCSF was lyophilized after extensive dialysis against 1xPBS pH 7.4. Greater than 96.0% as determined by1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized GMCSF in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50 as calculated by the dose-dependent stimulation of the proliferation of human TF1 erythroleukemic cells is typically 1-4 ng/ml.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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