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Glutathione S-Transferase pi 1 Human Recombinant
Product Name Glutathione S-Transferase pi 1 Human Recombinant
Synonyms Glutathione S-transferase P, GST class-pi, GSTP1-1, GSTP1, FAEES3, GST3, PI, DFN7.
Description GSTP1 Human Recombinant fused with a 37 amino acids His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 247 amino acids (1-210 a.a.) and having a molecular mass of 27.6kDa.The GSTP1 is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P09211
Amino Acid SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP YTVVYFPVRG RCAALRMLLA DQGQSWKEEV VTVETWQEGS LKASCLYGQL PKFQDGDLTL YQSNTILRHL GRTLGLYGKD QQEAALVDMV NDGVEDLRCK YISLIYTNYE AGKDDYVKAL PGQLKPFETL LSQNQGGKTF IVGDQISFAD YNLLDLLLIH EVLAPGCLDA FPLLSAYVGR LSARPKLKAF LASPEYVNLP INGNGKQ.
Source Escherichia Coli.
Physical Appearance and Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity The GSTP1 solution contains 20mM Tris-HCl buffer (pH7.0), 30% glycerol, 1mM EDTA and 0.1mM PMSF. Greater than 95.0% as determined by SDS-PAGE.
Application
Solubility
Biological Activity Specific activity is < 6.5 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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