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Aldose Reductase Mouse Recombinant
Product Name Aldose Reductase Mouse Recombinant
Synonyms Aldose reductase, AKR1B1, AR, Aldehyde reductase, Akr1b3, Aldor1, Aldr1, Akr1b1, Ahr-1, Ahr1, ALR2.
Description AKR1B1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 339 amino acids (1-316a.a.) and having a molecular mass of 38.1kDa.AKR1B1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Uniprot Accesion Number P45376
Amino Acid SequenceMGSSHHHHHH SSGLVPRGSH MGSMASHLEL NNGTKMPTLG LGTWKSPPGQ VTEAVKVAID LGYRHIDCAQ VYQNEKEVGV ALQEKLKEQV VKRQDLFIVS KLWCTFHDKS MVKGAFQKTL SDLQLDYLDL YLIHWPTGFK PGPDYFPLDA SGNVIPSDTD FVDTWTAMEQ LVDEGLVKTI GVSNFNPLQI ERILNKPGLK YKPAVNQIEC HPYLTQEKLI EYCHSKGIVV TAYSPLGSPD RPWAKPEDPS LLEDPRIKAI AAKYNKTTAQ VLIRFPIQRN LVVIPKSVTP VRIAENLKVF DFEVSSEDMA TLLSYNRNWR VCALMSCAKH KDYPFHAEV.
Source E.coli.
Physical Appearance and Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity AKR1B1 protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4), and 10% glycerol. Greater than 95% as determined by SDS-PAGE.
Application
Solubility
Biological Activity Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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