Protein Kinase Akt1/PKB alpha, Inactive enzyme Human Recombinant

Item#:

rAP-1647

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Product Name	Protein Kinase Akt1/PKB alpha, Inactive enzyme Human Recombinant
Synonyms	RAC-alpha serine/threonine-protein kinase, EC 2.7.11.1, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, AKT1, AKT, RAC, PRKBA, MGC99656, RAC-ALPHA.
Description	PKAkt1 is a glycosilated polypeptide having a molecular mass of 59.1 kDa, fused with a polyhistidine tag at N-terminus (to facilitate removal of Akt1 kinase from the reaction mixture).Inactive enzyme, suitable for negative control experiments or for phosphorylation as a substrate.Recombinant Protein Kinase B is purified by proprietary chromatographic techniques.
Uniprot Accesion Number	P31749
Amino Acid Sequence
Source	Sf9 insect cells.
Physical Appearance and Stability	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Formulation and Purity	PKAkt1 in 50mM Tris-HCl, 100mM NaCl, 1mM DTT, 25mM beta glycerophosphate, 50% glycerol, pH 8.5. Greater than 90% as determined by SDS-PAGE.
Application
Solubility
Biological Activity	No protease activity (Twinning test). The Specific activity is 235 U/mg.
Shipping Format and Condition	Lyophilized powder at room temperature.
Usage Statement	Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.