CRISPR-Associated Protein-9 Nuclease S. Pyogenes Recombinant

Item#:

rAP-1350

Product Name	CRISPR-Associated Protein-9 Nuclease S. Pyogenes Recombinant
Synonyms	CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, cas9, csn1.
Description	Recombinant Cas9-NLS produced in E.coli, comprises the entire Cas9 protein sequence (1368 amino acids) joined to a proprietary nuclear localization sequence (NLS) and a 6xHis tag at the C-terminus, having a total of 1414 amino acids. The protein has an apparent molecular weight of 163kDa.
Uniprot Accesion Number	Q99ZW2
Amino Acid Sequence
Source	Escherichia Coli.
Physical Appearance and Stability	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity	The CAS9 solution contains 10mM Tris-HCl, 300mM NaCl, 0.1mM EDTA, 50% glycerol and 1mM DTT pH 7.5. Greater than 90% as determined by SDS-PAGE.
Application
Solubility
Biological Activity
Shipping Format and Condition	Lyophilized powder at room temperature.
Usage Statement	Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.