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Tumor Necrosis Factor-alpha Human Recombinant, HEK


Item#:
rAP-0757
Size Price Quantity
2.0 ug $70.00
10.0 ug $182.00
1000.0 ug $7,280.00
Product Name Tumor Necrosis Factor-alpha Human Recombinant, HEK
Synonyms TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Description TNF-a Human Recombinant produced in HEK cells is a glycosylated non-disulfide linked homotrimer, containing 157 and having total Mw of 17kDa.The TNF-a is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P01375
Amino Acid SequenceVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL.
Source HEK.
Physical Appearance and Stability Lyophilized TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The TNF-a protein was lyophilized from 1mg/ml in 1xPBS. Greater than 95% as obsereved by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized TNF-a in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The specific activity was determined by the dose-dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D and is typically 0.05-0.5ng/ml.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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