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Interleukin-35 Human Recombinant


Product Name Interleukin-35 Human Recombinant
Synonyms
Description IL35 Human Recombinant produced in HEK 293 cells is comprised of a p35 subunit from IL-12 (Accession # p29459) and a EBI3 (Accession # q14213) subunit from IL-27. IL35 is a heterodimeric polypeptide chain containing 442 amino acids, with a predicted molecular weight of 49 kDa. From N terminus to C terminus, the molecule is comprised of a poly His tag, the EBI3 subunit, a G rich linker and the p35 subunit.
Uniprot Accesion Number
Amino Acid SequenceEBI3 subunit
Source HEK 293 cells.
Physical Appearance and Stability Lyophilized IL35 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL35 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Formulation and Purity Lyophilized from a concentrated solution containing 10mM Na2PO4 pH 7.5 and 30mM NaCl. Greater than 95.0% as determined by analysis by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized IL35 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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