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Interleukin-12 Rat Recombinant
Product Name Interleukin-12 Rat Recombinant
Synonyms NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.
Description Interleukin-12 Rat Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.The IL-12 is purified by proprietary chromatographic techniques.
Uniprot Accesion Number
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Ile-Pro-Val at the p35 subunit and Met-Thr-Glu-Leu-Glu at the p40 subunit.
Source Baculovirus.
Physical Appearance and Stability Lyophilized Interleukin-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL12 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The protein (1mg/ml) was lyophilized with no additives. Greater than 95.0% as determined by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized Interleukin 12 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity ED50 range = 0.01-0.05 ng/ml corresponding to a specific activity of 20,000,000-100,000,000IU/mg as determined by the dose dependent secretion of IFN-gamma from PHA and IL-2 activated mouse splenocytes that have been costimulated with PMA. Optimal concentration for individual application should be determined by a dose response assay.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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