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Interleukin-12 Mouse Recombinant
Product Name Interleukin-12 Mouse Recombinant
Synonyms NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.
Description Interleukin-12 Mouse Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 506 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.The IL-12 is purified by proprietary chromatographic techniques.
Uniprot Accesion Number
Amino Acid Sequencep35 Subunit
Source HEK-293 Cells.
Physical Appearance and Stability Lyophilized mouse IL-12 although stable at room temperature for 3 weeks, should be stored below -18°C. Upon reconstitution IL12 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The protein was lyophilized from 0.5xPBS, pH 7.5. Greater than 95% as determined by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized murine IL12 in sterile distilled pyrogen free water at 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity IL-12 Mouse has full biological activity when compared to standards. The ED50 is determined by the dose-dependent induced expression of CD25 on CD8+ mouse T cells is 10-20 ng/ml corresponding to a specific activity of 50,000-100,000units/mg.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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