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Flt3 Ligand Human Recombinant, Sf9
Product Name Flt3 Ligand Human Recombinant, Sf9
Synonyms Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.
Description Flt3-Ligand Human Recombinant produced in Sf9 insect cells is a glycosylated, polypeptide chain containing 155 amino acids and having migrating on SDS-PAGE at 19kDa. Flt3-Ligand is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P49771
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser.
Source Baculovirus Sf9 cells.
Physical Appearance and Stability Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-L should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The protein was lyophilized with no additives. Greater than 95.0% as determined by
Application
Solubility It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50, calculated by the dose-dependent stimulation of the proliferation of human MUTZ-2 cells is < 3.0 ng/ml.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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