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Bone Morphogenetic Protein-7 Human Recombinant, CHO

Product Name Bone Morphogenetic Protein-7 Human Recombinant, CHO
Synonyms Osteogenic Protein 1, BMP-7.
Description N-TERMINAL---Human BMP-2 (Met 1 ¿ Arg 282) Human BMP-7 (Ser 293 ¿ Arg 431)---C-TERMINAL. The DNA sequence encoding the human BMP-2 signal peptide and propeptide (1~282 amino acid) fused to the human rhBMP-7 mature chain (293~431 amino acid) was expressed in a Chinese hamster ovary cell line. The mature recombinant BMP-7 generated by the proteolytic removal of the signal peptide and propetide contains 139 amino acid residues. The glycosylation of BMP-7 increases the molecular mass and the glycosylated proteins migrate as 25 ~ 40 kDa in SDS-PAGE under non-reducing conditions. BMP-7 is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P18075

Amino Acid Sequence

Source Chinese Hamster Ovarian Cells.
Physical Appearance and Stability Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP 7 Human should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 1% sucrose, 1.2% mannitol, 20mM glycine and 0.05% polysorbate 20 pH-4.   Greater than 97.0% as determined by
Solubility It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-7 in 0.5mg/1ml sterile/ endotoxin free water.
Biological Activity Measured in alkaline phosphatase activity assay using MC3T3-E1 cells. The ED50 for this effect is < 70ng/ml, corresponding to a Specific Activity of 14,286IU/mg.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.