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GFP Expressing Mouse RAW246.7 Cells

Size Price Quantity
1.0 Frozen Vial $790.00
Name of Products GFP Expressing Mouse RAW246.7 Cells
Catalogue NumbercAP-m0101GFP
Product FormatFrozen Vial
Cell Number> 5x10[5] cells/vial
Mouse RAW246.7 Cells were established from a tumor induced by Abelson murine leukemia virus in a BALB/c mouse. GFP-RAW246.7 were selected from RAW246.7 cells infected with GFP expressing lentiviruses (under the control of CMV promoter) with puromycin. Universal Growth Medium (cAP-01B) is recommended for the expansion of GFP-RAW246.7 cells. And we warranty a minimum of 10 passages of the cells if cultured following the detailed protocol described below).
1.GFP-RAW246.7 cells are tested negative for common animal pathogen and mycoplasma.
The cells are offered for Research Use Only.
Frozen Vials in a Dry Ice Package.
When you receive the dry ice package with cells in frozen vials, transfer the frozen vials of cells into a -80C freezer for short period storage or a liquid nitrogen tank for long-term storage.
A)Pre-coating of T25 flasks- Add 2ml each Quick Coating Solution (cAP-01) into a T25 flask to cover the whole surface of the flask, 5 mins later, aspirating the excessive coating solution and the flask is ready to be used.
B) Thaw the frozen cell vial in a 37C water bath first, and then transfer the cells into the pre-coated T25 flask with 10ml of cAP-01B medium, cells usually become confluent overnight and ready to be passaged.
C) To passage the cells, rinse the cells in a T25 flask with 5ml HBSS (RT) twice; then add 2ml Trypsin/EDTA (RT) (cAP-23) into one T25 flask; gently aspirating the excessive Trypsin/EDTA solution within 20 seconds by aspiration.
D) Leave the T25 flask with the cells at RT or 37C for 1 min (most cells usually will detach from the surface within 1-2 mins; or monitor the cells under a microscope until most of the cells become rounded up, and then gently tap the flask against the bench surface, and the cells will move on the surface of the flask when monitoring under a microscope.
E) Add 5ml Trypsin Neutralization Buffer and spin down the cells with 800g centrifugation for 5 mins.
G)Re-suspend the cell pellet with 15ml cAP-01B medium and transfer 5 ml each into 3 pre-coated T25 flasks (for 1/3 subculture ratio).
H) Change medium every 2 or 3days and the cells usually become confluent within 5-7 days (when split at a 1/3 ratio).
Quick Coating SolutioncAP-01
Universal Growth MediumcAP-01B
HBSS w/o Ca2+, Mg2+cAP-11
Cell Freezing Solution (FBS)cAP-22
Cell Freezing Solution (Non-FBS)cAP-22B
Trypsin/EDTA SolutioncAP-23
Trypsin Neutralization SolutioncAP-28
ITS (100x)cAP-26
L-Glutamine-MAXIMUM (100x) cAP-27
Human Plasma Fibronectin SolutioncAP-42
Handling human and animal tissue derived products is potentially bio-hazardous. Although each cell strain is tested negative for HIV, HBV and HCV DNA, or pathogens, diagnostic tests are not necessarily 100% accurate; therefore proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working with these materials.