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Mouse Monoclonal anti-human FIH-1


Item#:
hAP-0997
Size Price Quantity
50.0 ug $420.00
Catalog Number hAP-0997
Mouse Monoclonal anti-human FIH-1
Size 100ug
Immunogen Recombinant human FIH-1
Clone MM0997-5W29
Antibody Isotype Mouse IgG2
Preparation This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells) from a mouse immunized with recombinant human FIH-1 (Factor-inhibiting hypoxia-inducible factor 1). FIH-1 is also called HIF-1AN and is a 40-45kDa oxygenase that hydroxylates asparagine, aspartate, and histidine residues in target proteins
Formulation and Storage The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70C
Specificity This antibody recognizes human FIH-1 in direct ELISAs and WBs. It cross reacts with mouse FIH- 1 in WBs.
Reconstitution Reconstitute the antibody with 500ul sterile PBS and the final concentration is 200ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B.
C
D.
N
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