Goat anti-BRD4 Antibody |
| Principle Name | BRD4 |
| Official Symbol | BRD4 |
| All Name and Symbols | BRD4; bromodomain containing 4; CAP; HUNK1; HUNKI; MCAP; bromodomain-containing 4; bromodomain-containing protein 4; chromosome-associated protein |
| Accession Number(s) | NP_490597.1; NP_055114.1 |
| Peptide Sequence | PETSNPNKPKRQTNQ, is from internal region (near N Terminus) |
| Human Gene ID(s) | 23476 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_490597.1; NP_055114.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 150kDa band observed in Mouse Testis lysates (calculated MW of 155.8kDa according to Mouse NP_065254.3). Recommended concentration |
| Reference(s) | Crawford NP, Alsarraj J, Lukes L, Walker RC, Officewala JS, Yang HH, Lee MP, Ozato K, Hunter KW. Bromodomain 4 activation predicts breast cancer survival. Proc. Natl. Acad. Sci. U.S.A. 2008 Apr 105 (17) |
