Goat anti-LIMK2 (aa181-193) Antibody

Item#:

dAP-3443

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-LIMK2 (aa181-193) Antibody
Principle Name	LIMK2 (aa181-193)
Official Symbol	LIMK2
All Name and Symbols	LIMK2; LIM domain kinase 2
Accession Number(s)	NP_001026971.1; NP_005560.1; NP_057952.1
Peptide Sequence	QVKEVNRMHISPN, is from internal region
Human Gene ID(s)	3985
Non-Human GeneID(s)	16886 (mouse); 29524 (rat);
Important Comments	This antibody is expected to recognize all reported isoforms (NP_001026971.1; NP_005560.1; NP_057952.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human, Mouse, Rat
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 75+80kDa doublet band observed in lysates of cell lines HeLa and NIH3T3 and approx 70kDa observed in Rat Pancreas lysates (calculated MW of 77.9kDa according to Human NP_001026971.1 and 72.2kDa according to Human NP_005560.1, Mouse N
Reference(s)	Goyal P, Pandey D, Siess W. Phosphorylation-dependent regulation of unique nuclear and nucleolar localization signals of LIM kinase 2 in endothelial cells. The Journal of biological chemistry 2006 Sep 281 (35)