Goat anti-Fumarase / FH, Biotinylated Antibody

Item#:

dAP-3350

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Fumarase / FH, Biotinylated Antibody
Principle Name	Fumarase / FH, Biotinylated
Official Symbol	FH
All Name and Symbols	FH; fumarate hydratase; FMRD; HLRCC; LRCC; MCL; MCUL1; fumarase
Accession Number(s)	NP_000134.2
Peptide Sequence	HPNDHVNKSQSSND., is from internal region
Human Gene ID(s)	2271
Non-Human GeneID(s)	14194 (mouse); 24368 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, EIA
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Approx 48kDa band observed in Human Kidney lysates (calculated MW of 54.6kDa according to NP_000134.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration
Reference(s)	Pollard PJ, Spencer-Dene B, Shukla D, Howarth K, Nye E, El-Bahrawy M, Deheragoda M, Joannou M, McDonald S, Martin A, Igarashi P, Varsani-Brown S, Rosewell I, Poulsom R, Maxwell P, Stamp GW, Tomlinson IP. Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway. Cancer cell 2007 Apr 11 (4)