Goat anti-dUTPase / DUT Antibody

Item#:

dAP-3272

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-dUTPase / DUT Antibody
Principle Name	dUTPase / DUT
Official Symbol	DUT
All Name and Symbols	DUT; deoxyuridine triphosphatase; dUTPase; dUTP nucleotidohydrolase; dUTP pyrophosphatase; deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
Accession Number(s)	NP_001020419.1; NP_001939.1; NP_001020420.1
Peptide Sequence	QLRFARLSEHATAPT, is from internal region
Human Gene ID(s)	1854
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognize all reported isoforms (NP_001020419.1; NP_001939.1; NP_001020420.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 20kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 17.7kDa according to NP_001939.1). Recommended concentration
Reference(s)	Tóth J, Varga B, Kovács M, Málnási-Csizmadia A, Vértessy BG. Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme. The Journal of biological chemistry 2007 Nov 282 (46)