Goat anti-VPS16 Antibody |
| Principle Name | VPS16 |
| Official Symbol | VPS16 |
| All Name and Symbols | VPS16; vacuolar protein sorting 16 homolog (S. cerevisiae); hVPS16; OTTHUMP00000030047; OTTHUMP00000030048; vacuolar protein sorting protein 16; vacuolar protein sorting-associated protein 16 homolog |
| Accession Number(s) | NP_072097.2; NP_536338.1 |
| Peptide Sequence | CYKVQQKDVSDED, is from internal region |
| Human Gene ID(s) | 64601 |
| Non-Human GeneID(s) | 80743 (mouse); 296159 (rat); |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_072097.2; NP_536338.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 95+80kDa band observed in lysates of cell line HepG2 and 95kDa in Mouse Spleen (calculated MW of 94.7kDa according to NP_072097.2 and 78.3kDa according to NP_536338.1). Recommended concentration |
| Reference(s) | Huizing M, Didier A, Walenta J, Anikster Y, Gahl WA, Krämer H. Molecular cloning and characterization of human VPS18, VPS 11, VPS16, and VPS33. Gene. 2001 Feb 21;264(2) |
