Goat anti-KAP1 Antibody |
| Principle Name | KAP1 |
| Official Symbol | TRIM28 |
| All Name and Symbols | TRIM28; tripartite motif-containing 28; FLJ29029; KAP1; RNF96; TF1B; TIF1B ; KRAB-associated protein 1; nuclear corepressor KAP-1; transcriptional intermediary factor 1-beta; tripartite motif-containing 28 protein |
| Accession Number(s) | NP_005753.1 |
| Peptide Sequence | KQFNKLTEDKADVQ, is from internal region |
| Human Gene ID(s) | 10155 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Approx 100kDa band observed in nuclear lysates of cell lines HeLa, Jurkat and K562 (calculated MW of 88.5kDa according to NP_005753.1). The observed molecular weight corresponds to earlier findings with different antibodies from other comme |
| Reference(s) | Sripathy SP, Stevens J, Schultz DC. The KAP1 corepressor functions to coordinate the assembly of de novo HP1-demarcated microenvironments of heterochromatin required for KRAB zinc finger protein-mediated transcriptional repression. Mol Cell Biol. 2006 Nov;26(22) |
