Goat anti-HES4 Antibody |
Principle Name | HES4 |
Official Symbol | HES4 |
All Name and Symbols | HES4; hairy and enhancer of split 4 (Drosophila); bHLHb42; bHLH factor Hes4; class B basic helix-loop-helix protein 42; hHES4; transcription factor HES-4 |
Accession Number(s) | NP_001135939.1 |
Peptide Sequence | RSAAEHRKVGSRP, is from internal region |
Human Gene ID(s) | 57801 |
Non-Human GeneID(s) | |
Important Comments | This antibody is expected to recognize isoform 1 (NP_001135939.1) only. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
Applications | Pep ELISA, WB |
Tested Species | Human |
Peptide ELISA | antibody detection limit dilution 1 to 128000. |
Western Blot | Approx 24kDa band observed in nuclear lysates of cell line HepG2 (calculated MW of 26.1kDa according to NP_001135939.1). Recommended concentration |
Reference(s) | El Yakoubi W, Borday C, Hamdache J, Parain K, Tran HT, Vleminckx K, Perron M, Locker M. Hes4 Controls Proliferative Properties of Neural Stem Cells During Retinal Ontogenesis. Stem cells (Dayton, Ohio) 2012 Sep |