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Goat anti-RPA1 / RPA70 (aa323-337) Antibody
Item#:
dAP-2924
Size Price Quantity
50.0 ug $310.00
Goat anti-RPA1 / RPA70 (aa323-337) Antibody
Principle Name RPA1 / RPA70 (aa323-337)
Official Symbol RPA1
All Name and Symbols RPA1; replication protein A1, 70kDa; HSSB; MST075; REPA1; RF-A; RP-A; RPA70; MSTP075; RF-A protein 1; RP-A p70; replication factor A protein 1; replication protein A 70 kDa DNA-binding subunit; single-stranded DNA-binding protein
Accession Number(s) NP_002936.1
Peptide Sequence CKSYEDATKITVRSN, is from internal region
Human Gene ID(s) 6117
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human, Mouse
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 70+80kDa band observed in lysates of cell line HeLa (calculated MW of 68.1kDa according to NP_002936.1). The upper band likely represents the acetylated protein. A 75kDa band was observed in several Mouse cell lines and in lysates of
Reference(s) Lada AG, Waisertreiger IS, Grabow CE, Prakash A, Borgstahl GE, Rogozin IB, Pavlov YI. Replication protein A (RPA) hampers the processive action of APOBEC3G cytosine deaminase on single-stranded DNA. PLoS One. 2011;6(9)

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