Goat anti-CSTF3 Antibody |
| Principle Name | CSTF3 |
| Official Symbol | CSTF3 |
| All Name and Symbols | CSTF3; cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa; CSTF-77; MGC117398; MGC43001; MGC75122; CSTF 77 kDa subunit; cleavage stimulation factor subunit 3; cleavage stimulation factor subunit 3, isoform 1 |
| Accession Number(s) | NP_001317.1 |
| Peptide Sequence | RETKGKLPSYKE, is from internal region |
| Human Gene ID(s) | 1479 |
| Non-Human GeneID(s) | 228410 (mouse); 362178 (rat); |
| Important Comments | This antibody is expected to recognize isoform 1 (NP_001317.1) only. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 75kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 82.9kDa according to NP_001317.1). The observed molecular weight corresponds to the expected size of 77kDa. Recommended concentration |
| Reference(s) | Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, S Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 2007 3 |
