Goat anti-TNNT1 Antibody |
| Principle Name | TNNT1 |
| Official Symbol | TNNT1 |
| All Name and Symbols | TNNT1; troponin T type 1 (skeletal, slow); ANM; FLJ98147; MGC104241; STNT; TNT; TNTS; slow skeletal muscle troponin T; troponin T, slow skeletal muscle; troponin-T1, skeletal, slow |
| Accession Number(s) | NP_003274.3; NP_001119604.1; NP_001119605.1 |
| Peptide Sequence | EKAQELSDWIHQLE, is from internal region |
| Human Gene ID(s) | 7138 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize all reported isoforms (NP_003274.3; NP_001119604.1; NP_001119605.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 37+35kDa bands observed in Human Skeletal Muscle lysates (calculated MW of 32.9kDa according to NP_003274.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Reco |
| Reference(s) | Midde K, Dumka V, Pinto JR, Muthu P, Marandos P, Gryczynski I, Gryczynski Z, Potter JD, Borejdo J. Myosin cross-bridges do not form precise rigor bonds in hypertrophic heart muscle carrying troponin T mutations. J Mol Cell Cardiol. 2011 Sep;51(3) |