Goat anti-EAAT1 / GLAST1 Antibody

Item#:

dAP-2791

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-EAAT1 / GLAST1 Antibody
Principle Name	EAAT1 / GLAST1
Official Symbol	SLC1A3
All Name and Symbols	SLC1A3; solute carrier family 1 (glial high affinity glutamate transporter), member 3; EA6; EAAT1; FLJ25094; GLAST; GLAST1; GLAST-1; OTTHUMP00000116002; excitatory amino acid transporter 1; sodium-dependent glutamate/aspartate transporter 1; solute carrie
Accession Number(s)	NP_004163.3; NP_001160167.1
Peptide Sequence	DNETEKPIDSETK, is from C Terminus
Human Gene ID(s)	6507
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognize isoform 1 (NP_004163.3) and isoform 2 (NP_001160167.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Approx 60kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 59.6kDa according to NP_004163.3). Recommended concentration
Reference(s)	Vandenberg RJ, Handford CA, Campbell EM, Ryan RM, Yool AJ. Water and urea permeation pathways of the human excitatory amino acid transporter EAAT1. Biochem J. 2011 Oct 15;439(2)