Goat anti-POU4F2 Antibody

Item#:

dAP-2783

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-POU4F2 Antibody
Principle Name	POU4F2
Official Symbol	POU4F2
All Name and Symbols	POU4F2; POU class 4 homeobox 2; BRN3.2; BRN3B; Brn-3b; Brn3b POU domain transcription factor; POU domain class 4 transcription factor 2; POU domain protein; POU domain, class 4, transcription factor 2
Accession Number(s)	NP_004566.2
Peptide Sequence	HGLPSHMGCMSDVD, is from internal region
Human Gene ID(s)	5458
Non-Human GeneID(s)
Important Comments	This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459)
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Preliminary experiments gave an approx 70kDa band in Rat Retina lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.2k
Reference(s)	Qiu F, Jiang H, Xiang M, A comprehensive negative regulatory program controlled by Brn3b to ensure ganglion cell specification from multipotential retinal precursors. The Journal of neuroscience