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Goat anti-ARPC1A Antibody
Item#:
dAP-2605
Size Price Quantity
50.0 ug $310.00
Goat anti-ARPC1A Antibody
Principle Name ARPC1A
Official Symbol ARPC1A
All Name and Symbols ARPC1A; actin related protein 2/3 complex, subunit 1A, 41kDa; Arc40; SOP2Hs; SOP2L; OTTHUMP00000206034; SOP2-like protein; actin binding protein (Schizosaccharomyces pombe sop2-like); actin-related protein 2/3 complex subunit 1A
Accession Number(s) NP_006400.2; NP_001177925.1
Peptide Sequence DFKTLESSIQGLR, is from C Terminus
Human Gene ID(s) 10552
Non-Human GeneID(s) 56443 (mouse); 81824 (rat);
Important Comments This antibody is expected to recognize both reported isoforms (NP_006400.2; NP_001177925.1). No cross-reactiviry expected with ARPC1B.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Mouse, Rat
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Approx 45kDa band observed in lysates of cell line HeLa (calculated MW of 41.6kDa according to NP_006400.2). Approx 37kDa band observed in fetal Mouse Brain and adult Rat Brain lysates (calculated MW of 41.6kDa according to Mouse NP_062741.
Immunohistochemistry Paraffin embedded Human Heart. Recommended concentration
Reference(s) Laurila E, Savinainen K, Kuuselo R, Karhu R, Kallioniemi A. Characterization of the 7q21-q22 amplicon identifies ARPC1A, a subunit of the Arp2/3 complex, as a regulator of cell migration and invasion in pancreatic cancer. Genes Chromosomes Cancer. 2009 Apr;48(4)

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